Yes...trash all the current bedding. You can put the birds back in the coop in about 30 mintues or so.Thank you!!! When you spray this, do you remove all bedding first, and and should you let it sit? I can completely clean out the coop, but I don’t where to house the girls at night if it needs to sit, and then how often would I be completely removing all shavings and hay? Thanks again!