PoultryParadise, I wouldn't bother separating them (unless you're really bored), it won't help at all with standard mealworms since their cannibalism rate is so low (some of the larger species of mealworms are more cannibalistic than T. molitor and pupae need to be separated). If you look at the colony on my page you'll see a vigorous, healthy colony, one that is over 20 years old (that colony was split off another that was equally as old). I've never separated worms, pupae and beetles. Clearly, my colony doesn't suffer for it. I also try to keep some vegetable matter with moisture content remaining inside at all times, although they'll survive just fine if you forget for a few weeks (even months). Just monitor your colony, if it starts getting stinky, you've added too much moisture. You'll quickly see what they need. Good luck!