It depends on the breed and sometimes even the individual roo.
I would suggest that you not seperate them at all. As they get older they will spar and estabilish a pecking order. They may still fight from time to time but usually each one will know his place.
This might not be true with some birds. They may want to fight all the time even until death.
If you ever separate them and then put them back together, they are much more likly to have some serious battles.
Also one rooster to 23 hens is a little much. It should be no more than 12 to 14 hens per rooster and that may be too many depending on your roo.
If they are different breeds, you might want to pair them with the same breed hens when they are older in order to keep a pure line of the breed. Or what ever crosses you want to create.
Of course that means separate pens.
I currently have four roosters in my main pen and they get a long fine as long as each one minds his place. They do still test each other. If I would put another rooster in with them now, it would be chaos and all five would go at each other!
I hope this helps.