I believe after the eggs have been in the incubator for 20, 24 hour days. So if you put them in at 8 pm on a Sunday, the next Sunday at 8 pm will be 7 full days, the next Sunday at 8 pm would be 14 full days, and the next Saturday at 8 pm would be 20 full days, 8:01 pm on that Saturday would start day 21 and day 21 would end at 8 pm on Sunday.