When to open up the neat boxes?

My current flock of girls just turned 16 weeks this week. I had put the nesting boxes in about 2 weeks ago, so this week I opened them up and put some golf balls in the nests. Now, I just have to wait for them to start giving up the eggs. I have a mixed flock of BA's, PBR's, Red Stars, and Black Stars. I've noticed some of their faces getting red but no real growth on the combs.
 
I opened mine early, not for any particular reason.
Mine were about 9 weeks when I opened them, no issues with them sleeping in it.
I have red sexlinks that are 14.5 weeks. Still too young to be laying.
 

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