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Feather loss is one indication of mites. Until you actually observe the feather picking, you won't know for sure it's not caused by mites. Yes, you will need to treat both birds and coop.

If you have coop mites, they hide in cracks during the day and come out at night to feed on your chickens. Check at night by wiping a damp paper towel over the roosts. Check during the day to see if there are anything crawling on the skin of your hens around the vent. DE will do nothing to...

Have you examined the coop and nests for parasites? That can cause as much stress to hens as mosquitoes cause us to want to scream in frustration. Mites can also cause anemia in hens which would affect laying in addition to the stress.

Read this about chicken sex change. https://www.livescience.com/13514-sex-change-chicken-gertie-hen-bertie-cockerel.html Stress to one chicken can affect them while similar stress won't phase another. I have two different breeds of baby chicks right now, and the Andelusians are skittish while...

If Tree-puff laid through the winter months, she may not have molted, and is getting ready to do so now. Do you think this is a possibility? A molting chicken will have a solid patch of emerging pin feathers in balding places. Check her for this. Laying can be interrupted by stress, diet...