Yes. I only have one rooster and I've never used this incubator before so i didn't want to get anything precious. My rooster is lavender ameraucana and there's some welsummer, buff orpington, lavender ameraucana, ee, and a few japanese bantam eggs in there that i don't think will hatch. I have had my little banty mostly isolated from the rest of the girls. They pick on her too much.