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OK, I'm going to say it, most of us are sitting here, shaking our heads... I understand a bit of what you're saying about the animals being here (there) first, but that doesn't mean we need to hand them a dinner reservation either. You seem to be planning for keeping them safe, but your chickens are prey. Plain and simple.
Housecats rarely consider hens food, they'll leave them alone most likely, FERAL cats might try, but are rarely successful.
Coons will climb over, dig under, open latches with their cleaver little fingers, or tear/chew wire to get to hens and eggs, they can also reach in through holes and wire openings and rip heads, legs, wings or just chunks out of a hen, eating what they can reach bit by bit. (Gross I know, but it's the hard truth)
Opossums will dig under, and will eat what they can, drag it out of the pen if they can as well...
Coyotes will kill every one if they can for the joy of killing, then carry one or two off to enjoy at their leisure.
Neighborhood dogs will look at them as toys, flappy noisy toys that are fun to chase, often killing is just an accident, but sometimes it will give them a taste of what it's like to return to being a predator as their ancestors once were... some breeds are closer to their predatory ancestors than others, but either way, your hens will be just as dead, be it toy or dinner.
Fox are also clever, and they are more common than most people realize, they are spreading fast throughout the country, and are every bit as adaptable to human settlements as are possums, raccoons, coyotes.
You also mentioned putting up some protection across the top of the run against winged predators, they too will be eying the chicken dinner buffet you are presenting them, but you seem to be taking that into consideration too...
Anyway, I'm not meaning to sound negative, I live where we have all the same predators, heh. It's all part of the joy of keeping hens! They don't all descend on the coop en mass for supper, but its good to plan for the dangers that are there.