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Hubby and I had a discussion about that a while ago, I just never did research it! Not that I want to incubate my different breeds X my one only SLW roo! LOLwell comparing all 4 eggs that I have cracked 2 had no visible white spot and the other 2 had the white spot that looked like a bulls eye. These have not been incubated but straight from the nest to the fridge. All of the pics I have seen have been day 1 as well. Good question. Now I am wondering...