I learned the refrigeration method from an actual scientific study, & have been using it since. According to the study, the female chicks are genetically Male, & when bred to a rooster they will produce only males.How?
If a male embryo has ZZ sex chromosomes, I do not see any way refrigerating it can make the chromosomes become ZW. Cold temperature is not going to add something that was not there before (W chromosome.)
(And yes, it is very clear that chicken gender is determined by the chromosomes. All those sexlink hybrids are based on it, and they are so common we know it works.)
That's great! But if you have no dead embryos, it means you are not doing anything that kills male embryos.
I think the eggs were only refrigerated for a few days before incubation though.