EDUCATIONAL INCUBATION & HATCHING CHAT THREAD, w/ Sally Sunshine Shipped Eggs

@Sally Sunshine OK I made a little reaserch this is my conclusion.
In a dog vaginal smear you can find some different kind of cells:
Parabasal cells
1602996099329.png

They are the smallest epithelial cells (small cell with round nucleus the round purple dot in the cell)
Intermidiat cells
1602996270876.png

They are large(2,3 time more then the first one) fairly round with a nucleus.
Superficial cells
1602996538575.png



Large cells not round but polygonal usually without a nucleus and they can be found also in large sheets.

1602996612171.png

Red Blood cells
Very smal cells
1602996954887.png

The small red/ purple red dots in this pic.
Whit blood cell( Neutrophils)
Cells with a nucleus that isn't round but lobbed
1602997132276.png

And some bacteria
Wait for the second post.
 
@Sally Sunshine
In the Canine Estrous Cycle there are 4 stages
Anestrus
Intermediate and parabasal cells predominate in smears taken during anestrus. Superficial cells are absent or found in very small numbers. Neutrophils may also be present or absent.
See the pic
1602997648869.png

Proestrus
Serum concentrations of estrogen rise during proestrus, leading to capillary breakage and leakage of red blood cells through uterine epithelium, as well as proliferation of the vaginal epithelium.

Examination of vaginal smears from early to late proestrus will reveal a gradual shift from intermediate and parabasal cells to superficial cells. Typically, red blood cells are present in large numbers and neutrophils are commonly observed. Large numbers of bacteria are also often present.
See pictures

1602997863623.png


Estrus
The defining characteristic of cytologic estrus is the predominance of superficial cells. Most, but not all, bitches will undergo full cornification, and the smear will reveal a monotonous pattern composed almost exclusively of anucleate superficial cells.
1602997959177.png

Diestrus
The onset of diestrus is marked by a precipitous decline in the number of superficial cells and reappearance of intermediate and parabasal cells. Most commonly, the cellular profile changes within a single day from essentially 100% superficial cells to less than 20% superficial cells. However, it is best to confirm the onset of diestrus by examining a smear prepared on diestrus day 2.

1602998068984.png

For good chance for conception your dog have to bee on eastrus,
1602998215585.png

So that smear need to have a majority of Superficial cells!
Good luck.
 
This beauty is now in the US.....
View attachment 2377282
Shalom!
I really like that knife. Would you remind me of my math? I need to give dexamethasone to a llama that somewhere between 170 and 200 lb at the moment I would guess at 0.1 ml per kilogram. could you remind me what equation I need to use? it has been too long not to mention I don't use kilograms much I know it's 2.2 lb so I could divide. is it essentially one cc per 22kg? since I need to give dexamethasone I want to be right.
Thanks!

@casportpony @Sally Sunshine
 
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Shalom!
I really like that knife. Would you remind me of my math? I need to give dexamethasone to a llama that somewhere between 170 and 200 lb at the moment I would guess at 0.1 ml per kilogram. could you remind me what equation I need to use? it has been too long not to mention I don't use kilograms much I know it's 2.2 lb so I could divide. is it essentially one cc per 10 kg? since I need to give dexamethasone I want to be right.
Thanks!

@casportpony @Sally Sunshine
0.1ml =1kg=2.2lb
= 0.05ml for 1.1lb
0.05ml:1.1lb= 1lb
=0.045ml=1lb
170lb=0.045ml×170=7.65ml
200lb=0.045ml×200=9ml
 
0.1ml =1kg=2.2lb
= 0.05ml for 1.1lb
0.05ml:1.1lb= 1lb
=0.045ml=1lb
170lb=0.045ml×170=7.65ml
200lb=0.045ml×200=9ml
the more I thought about it it's like I know that's a 10th per kilogram right yeah somehow I get nervous when I start dealing with meds and numbers especially when I am dealing with a steroid or med you have to be right with.
 
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