I am so glad you are doing this and recording it.
The first time I hatched eggs, I did not wash them. My incubator was really nasty after the hatch.
Now, I wash all the eggs. I've always had from 90 - 100 % hatch on my eggs. I count all eggs in my percentages. If it goes in the incubator, it is counted, even if it turns out to be infertile.
I hatch approximately 3000 chicks a year, and I wash every egg that I gather.
I don't use anything but water with mine, though. I don't let them sit in water, I just rinse them off one at a time, and use a soft brush like you, to rub off any mud or poop.
I have thought about using some sort of disinfectant, but was not willing to experiment, since just the plain water has worked for me.
I know the big hatcheries do disinfect the eggs before incubating them, but I don't know what they use for that.
Thanks for the formula you are using, I may just try it myself now.
Have you had any health issues with the chicks? That was always my main concern for not using a disinfectant.
Jean