Some hens are very territorial around their nests and will chase anything that gets within 2-3 feet of the nest.
If the enclosure isn't big enough to allow the other bird(s) a reasonable amount of space to move on away from the nest, then some kind of separation can be necessary. However, if you do separate them, and in particular if you do so by moving the male to an enclosure where they can't see each other clearly, the female will likely perceive him as a threat if you introduce him once the chicks have hatched.
If he hasn't had chicks before, he might actually BE a threat - some roos have no clue what to do about chicks and might peck them. The same actually goes for hens, but their hormones after being broody are more likely to tell them what to do.
The hen could however also accept him back once the chicks have hatched - and if the separation hasn't been too long, the roo might not be so desperate to breed that he chases her around. But there is no way to tell exactly what will happen.
If I were you, I'd keep them separated but as near each other as possible, for now. If it's possible to divide their current enclosure (without creating gaps a chick could get stuck in) with a single layer of very fine wire mesh, that would be the best way to keep them near each other.
Otherwise, just do the best you can and then try to introduce him once the chicks have hatched. If the hen won't accept him, if he chases her or or if he pecks the chicks, remove him and try again a couple of weeks later. By then the chicks are much more hardy and don't need mom much anymore, so they can take a few pecks and won't be too affected if mom chases the roo a bit or gets chased a bit herself. Just don't leave them unsupervised unless you are completely certain they'll be okay.