Genetically, will it matter if this turns out to be a pullet or cockerel?
It will only matter if you are working with genes on the Z sex chromsome.
Those would include:
gold/silver
barring
dark vs. light legs
chocolate
If all the birds involved are gold, and not-barred, and not-chocolate, then you don't have to think about those genes. They will keep breeding true for those traits.
If you want dark legs and not light ones, the genders will matter a bit. You can work with anything, but figuring out the details can make it go more smoothly.
You understand about chicken sex chromsomes, right? A hen only has one Z chromsome, so she shows whatever is on it. She inherits it from her father and passes it on to her sons. A rooster has two Z sex chromosomes, one from each parent, and gives one to each chick. Light legs are dominant over dark legs, so a dark-legged rooster must be pure for that recessive gene, while a light-legged rooster may carry the gene for dark (if he got it from his mother or from his father.)
If you breed dark legs x dark legs, all chicks will have dark legs.
A pure light-legged rooster x light-legged hen produces light legs.
If you cross the leg colors, sons will show light legs while carrying the gene for dark legs, while daughters will match their father.
A light-legged rooster who carries the gene for dark legs will produce 50% light-legged daughters and 50% dark-legged daughters, no matter what leg color their mother has. With a dark-legged hen he will produce 50% dark-legged sons and 50% light legged sons who carry dark (like himself.) With a light-legged hen he will produce only light-legged sons, but half will carry the gene for dark legs and half will be pure for light legs.
I would expect Cochins to have light legs, with Sebrights and Polish having dark legs, but looking at your own birds will be more accurate than me guessing from what the breeds are "supposed" to have.