There are two basic issues. Where are they going to lay eggs and where are they going to put themselves to bed to sleep. You could have issues with either of these even if they had not been in a tractor. You have two different ways you could work this.
If your coop is big enough and weather, especially hot weather, is not an issue I'd probably lock them in the coop section only for a few days. How long? I don't know, two days might be enough, a week might not work. I'd want it to be long enough that most if not all have laid an egg in there before I let them out. I don't know if they were roosting or sleeping on the ground while in the tractor. I'd feel better about letting them out if they were roosting at night in the coop instead of sleeping on the ground
You could just turn them loose in the coop/run combination and see what happens. It is certainly possible you'll be happy with the results or you may have to work to change where they sleep or lay. You never know what they will do, but I think your odds of being happy are better if you can lock them in the coop for a few days.
