How long will a hen stay bred once isolated?

aside from incubating, crack it open, look in the center of the yolk, there is always a whitish dot in the center, this is where the sperm will enter at, if it has a ring around the dot, it's fertile.
And usually 2 weeks is all you'll need, especially if placed with a new roo, as she will take the new deposits from him and usually discard any from old rules. Breeders use the 4 week rule when selling eggs or chicks to ensure there are no possible mixes going to their customers. If this is just for your own pleasure, hatch 'em, you'll be able to tell if they are pure or not when they hatch or feather up.
 

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