Vinnie, you can use the hardboard, heck any hardboard, used stuf toof, just paint it if need be .OSB or plywood painted too, ask at your local "Lumber Yards" for top sheets I just bought 2 for like $10.00.
As for rodents and insects, seal all, make sure you have tight fits on corners, possibly clad entire bottom of floor joists, and raise off ground, got any cats that can go under and take care of the meeces.
Insulation may not be 100% necessary in you jurisdiction, but it is nice for holding heat, and keeping out the heat of summer, not sure if insulating just the ceiling might be a good bet in you area,if you are not planning on ceiling insulation, forget about the walls, it won't be much value with any rising heating going out the roof. Create adequate venting, eliminate drafts, good dry bedding, correct volume of chickens, all should be good. I hope all works out, I cannot see our relatives a few generations back, being so concerned about chicken coops, but survival and running the farm was important, it was not a hobby for those folks...