45% is not extremely high, but if the eggs did not loose enough moisture, then, yes, it could have been lowered a bit.Would that account for such small Air cells going into lockdown??
Is one hour sufficient amount of time to compare the two readings?
For reference... I use this one... that was suggested by many BYC members.
http://incubatorwarehouse.com/incubator-remote-thermometer-hygrometer.html
Personally I say yes, it should be well over enough time to compare. It should not take that long after closing the lid for the hygrometers to catch up to the change of humidity.