Mites

Probably not. However, to be absolutely sure, it wouldn't hurt to dust them a third time at the 14 day interval to be doubly sure you have broken the mite lifecycle. You see, the dust will certainly kill live mites, but not necessarily the eggs. When you retreat them 14 days later, you are doing so to kill any newly hatched mites before they can mature and lay more eggs.
 
On the mite subject. My mom use to breed Persian cats and used religiously Boric Acid powder for their ears. the vet told her that her cats ears were the nicest free from scratches and cleanest of any of his clients and was wondering what she did. I was thinking of using Boric Acid in powder form on my chickens as a preventative measure. Just wanted to share the thought. Have a great holiday!
 
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No, just until you don't see any bugs. You can wash the feathers to remove some of the ugliness. Some of it is mite excrement, not eggs. That will wash away, but the eggs are sort of cemented to the feathers and you probably will just have to live with those until the next molt.
 

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