Do you spend much time observing the flock as they lounge around during the day? Do you see the interaction of the suspected bully with the other rooster, and the hens? I think you will see that the evidence is right in front of you if you watch for a while.
But then, if, as many suspect, your fully clothed roo is the culprit, you will need to make a decision as to what to do with him, once you quarantine him. The other roo will probably take over. He may be less rough with the girls, but he may be rougher. Yikes! It could even be that one of the older hens takes over as boss. She might boss that second rooster around.
I don't want to sound like a chicken whisperer here, but the dynamics of the flock will change once the one roo is taken out, and they will change again when you integrate the new girls. Just be aware that it may take a while to settle down. Make sure there are plenty of places for the younger ones to get out of the way of the older ones.
But then, if, as many suspect, your fully clothed roo is the culprit, you will need to make a decision as to what to do with him, once you quarantine him. The other roo will probably take over. He may be less rough with the girls, but he may be rougher. Yikes! It could even be that one of the older hens takes over as boss. She might boss that second rooster around.
I don't want to sound like a chicken whisperer here, but the dynamics of the flock will change once the one roo is taken out, and they will change again when you integrate the new girls. Just be aware that it may take a while to settle down. Make sure there are plenty of places for the younger ones to get out of the way of the older ones.