older pullets quit roosting

KLC321

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3 of my pullets started spending the night in nesting boxes. They are over 2 years old and have previously used the roosts. They have 3 different roosts.
 
Are they molting? Have you checked the roosts for red mites in the evening?
Thanks for your response. They are not molting. I put diatomaceous earth powder in their coop recently on the floor, nesting boxes and perches. I don't think I have red mites but will check tonight.
 
Has anything changed in the coop? Air blowing on the roosts? Leaky roof or water blowing in? Mice?
Has their flock order changed?

Pullet = female chicken under 1 year of age
Hen = 1+ year old
Cockerel = male chicken under 1 year
Rooster = 1+ year old
Yes definitely hens, not pullets. Everything is the same for the last 8 months or so. It doesn't seem like the flock order has changed - it is very quiet and they all seem to get along during the day. We had lots of drama and strife when we had a rooster but he's been gone for awhile and everything is calmer.
 

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