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A thermocouple and a heater. Both wire directly into the PID controller, providing you aren't using too big a heater.
That is where the instructions gave me a headache. They try to explain everything it can do. So for an incubator application 4 wires Twigg??
Seven wires ... you need to power it.
The instructions are complex because the unit is capable of far more than we want it to do. Just take what you need, and leave the rest.
For example ... they can usually be set to run in three entirely separate modes .... On/Off, Proportional and PID.
Most incubators use simple On/Off thermostats. Better incubators use proportional and commercial incubators use PID. The benefits are as follows:
On/Off is rough and ready, but it works.
Proportional is waaaaaay more stable
PID is proportional, but also adjusts for varying room temps.
I would set it up as proportional first, play with the settings and explore other features when you feel more comfortable.