I called and talked to three state vets; none of them seemed too concerned about it and said even if the chicks that died did have Pullorum, they doubted it was the cause of death or illness for any of the other animals-- they also said that if I really wanted they could test one of the chicks anyway though. However, their method of testing involves killing the chick first and then doing a necropsy, something I am not willing to do if not absolutely necessary...I've had too many deaths already, I don't want to be the cause of another, especially if it isn't needed.
After that I called my vet again and asked if they could test for it, which they said they could, but only with a blood sample, not a fecal sample. When I asked if they could get a blood sample without having to kill the chick first she told me she didn't know (the vet was gone by that point). I was getting the information from a secretary who in turn was getting the information from a vet (you apparently can't talk to the vets directly for some reason) and the secretary had never heard of Pullorum so I'm not totally sure how reliable the info I got was, but I did get a number for a state university that apparently does these kinds of tests on a daily basis so I'll call them when they're open on Monday and see what they say. I'm hoping they're able to do it without needing the chick to be dead first.
Does anyone here know anything more about testing for Pullorum? Is it absolutely necessary that the bird needs to be dead in order to perform the test? And if not, is there really no other way to test for it other than with a blood sample? I don't see how or where you could draw blood from those little chicks without causing some kind of serious damage, but then again I'm no expert with this stuff.
Finally, on a positive note, the initial reason I posted this thread (the two adult hens that looked very sick) seems to have mostly resolved itself. The Golden Lakenvelder has her eyes completely open now and looks fine; the Red Star still looks a little funny and her eyes still aren't fully open and her butt isn't fully clean either but she still looks better than before. Still have no idea what was going on with them that day, but whatever it is, it appears to be getting better.