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Could you explain why so I can look for other clues? I know it is hard to tell just by pictures but I have more hen/roo guesses than both roo guesses.
Coloring is a big indicator - males tend to have smut (bleed through colors, especially in the wing area) and overall have more "flashy" colors than females.
Comb - boys get wider/redder combs early on with the 3 rows being very prominent. Occasionally girls can pop the 3 rows early on (I've had a couple) but generally they appear to have a single ridge until older.
Legs - males have thicker legs.
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Could you explain why so I can look for other clues? I know it is hard to tell just by pictures but I have more hen/roo guesses than both roo guesses.
Patchy coloration. Girls tend to be patterned while boys tend to be patchy/splotchy/calico. The second one is definitely less sure because the comb hasn't developed yet but the color is male.
I have an 8 week old Americauna who I suspect is male (just had to give a male away two days ago after hearing the undeniable cock-adoodle-do. the remaining one has not crowed yet and my hope is that she wont. We replaced the cockeral with a Blacktailed Japanese Bantam who is 5 weeks old and after a brief introduction it appeared that the americauna was trying to mate with her (?) I admit I am new to the chicken scene but it looked pretty odvious - am i missing something? Do pullets imitate sex with other pullets? Pardon my ignorance but i really dont know and if she is really a he I need to rethink keeping "her". We have them seperated for now but they can see and hear one another (split the coop) until we can determine the sex of the americauna. I know that some pecking will be part of the introduction. Advice?
To answer JoeContreras' original question, the two EEs have male coloration, and the white and black one has a very male comb.
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To answer your question, yes some pullets do engage in this behavior as a dominance display. Most of the time it just involves hopping on top of the other hens, but it can get more extreme.
I'd keep an eye on her, 8 weeks is much too young to be 100% certain of Ameraucana gender.
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My 9-week EE cockerel and several pullets in the 7-10 week range display "male" behaviors -- jumping on backs, flaring necks, flying at another chicken with feet up. They're sexually immature and still working out the pecking order. At this age, I believe that is what you are seeing.