I have took them out when i got ready. I just spray the eggs to replace the miosture lost it won't hurt anything. I have been hatching eggs for many years and i read all these posts where people blame bad luck on opening the incubator. I just don't think that is the problem as long as you don't let your humidity get low and the membranes dry out. After all that's what the extra humidity is for. If you notice the membranes turning yellow that's when you need to spray them.