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could they be laying eggs in hiding, when you let them free range? Are their combs getting redder? Do they squat when you touch them on their backs?
Those are typical in a hen getting near point of lay.
She may just be playing the role of the rooster. Do you have a rooster? If you didn't that means that 1 of your chickens need to take over the flock and to be the top dog and the leader. I had the same problem. Except my littlest hen is the leader and top dog.
Hope this helps!