Yes, each state can be somewhat different. The basics seem to be standard, but several states require testing for additional things, besides the basics. I think they use at least 2 reagents when they do the "field test" on mine. They put a drop of reagent on one end, then another drop of another one, on the other end. When they collect the little blood sample, they put a tiny bit on each end of the slide, for each bird, and save some to take back to the lab. They give a temporary letter, saying you passed your NPIP. The official results come back in a week, or two, with the card. (Sometimes it can take a bit longer, if the lab is really backed up.)