Yes, its kind of a bark. Now that you say females, the only male I had did not make this sound but he is gone now.Mine bark, is that the sound that you are talking about? None of my brahma females make normal chicken noises, they all just kind of bark when they need something or want someone to come over and look at something. If not that, if they don't seem to be in distress, I'd not worry overmuch about it.