Yes, air cell, which is generally on the fat end of the egg should go up in the turner or carton, whatever you are using as a holder, some eggs are hard to see the difference because they are slender on both ends or completely round, these you would need to candle to find the air cell, the air cell is fairly easy to find even with a low quality light most any flashlight should work if you make a cone to concentrate the light into a small spot, I have found I do need a better light to actually see chick development. If they are upside down it won't necessarily kill them, even eggs which are positioned properly sometimes have chicks positioned upside down inside the egg and they will pip at the wrong end, sometimes they hatch sometimes they don't. So it will improve your odds to have the eggs positioned properly but if they aren't it isn't a guaranteed loss.