Fecal Float Slide

[ZedikerStation]

Yes there is more than one type but the article is correct, no trichuris in chickens.

this is a common spurious find in fecals and had been confusing student parasitologist for centuries.

I'll keep looking!
We haven't found any worms yet then....

 

[theoldchick]

I thought that first, but read an article that said chickens don't get Whipworm. is there more than one type of Trichuris?

Yes. Trichuris can infect many species. However, chicken feces will show certain parasite eggs not because they have the parasite but because they have eaten matter that contained the microscopic eggs.

 

[Sue Gremlin]

Yes. Trichuris can infect many species. However, chicken feces will show certain parasite eggs not because they have the parasite but because they have eaten matter that contained the microscopic eggs.

eggzackly.

This is why sometimes we find pinworm eggs in cat fecals, even though cats don't get pinworms. But mice do.

 

[Sue Gremlin]

This image makes me think Trichuris View attachment 2822921

Stock photo:
View attachment 2822917

I wanted to send you special snaps for your reasoning here. Those little polar plugs are what should give anyone doing chicken fecals a little fright.
The subtle differences can be seen in the overall shape of the egg, but most importantly, in the size of the egg. (see left side of the photo, attached). The capillaria egg will be quite a bit larger than the monocystis. This is all well and good until you don't have them side-by-side, and you try to measure an egg under a microscope. It can be tricky, but often exact measurements are important to properly ID structures.
The good news is that some cameras do that automatically, which is something I super want because I am lazy. But as it is now, if you have a reticle (an eyepiece with a scale) you can use that to measure things. (you need to work out specifically what each mark on your reticle measures in mm, every microscope is different, but once you do that, it's relatively simple).

 

[ZedikerStation]

I wanted to send you special snaps for your reasoning here. Those little polar plugs are what should give anyone doing chicken fecals a little fright.
The subtle differences can be seen in the overall shape of the egg, but most importantly, in the size of the egg. The capillaria egg will be quite a bit larger than the monocystis. This is all well and good until you try to measure an egg under a microscope. It can be tricky, but often exact measurements are important to properly ID structures.
The good news is that some cameras do that automatically, which is something I super want because I am lazy. But as it is now, if you have a reticle (an eyepiece with a scale) you can use that to measure things. (you need to work out specifically what each mark on your reticle measures in mm, every microscope is different, but once you do that, it's relatively simple).

Are the reticles in the eye piece of the microscope? I have 3 extra 10X still in the box. Never looked at them. Perhaps one has it.... LOL

 

[Sue Gremlin]

Are the reticles in the eye piece of the microscope? I have 3 extra 10X still in the box. Never looked at them. Perhaps one has it.... LOL

Yes, they are part of the eyepiece. Scopes don't automatically have a reticle, you may have to purchase. I think mine was about 20 bucks on Ebay, but be careful, not all eyepieces will fit all microscopes, they are all different unfortunately.

The eyepiece just pulls right out, and the reticle can be dropped in its place.

 

[ZedikerStation]

Yes, they are part of the eyepiece. Scopes don't automatically have a reticle, you may have to purchase. I think mine was about 20 bucks on Ebay, but be careful, not all eyepieces will fit all microscopes, they are all different unfortunately.

The eyepiece just pulls right out, and the reticle can be dropped in its place.

I thought it was odd that it came with 3 additional eyepieces. Maybe one has it. I'm going to dig out the box!

 

[ZedikerStation]

I thought it was odd that it came with 3 additional eyepieces. Maybe one has it. I'm going to dig out the box

Yes, they are part of the eyepiece. Scopes don't automatically have a reticle, you may have to purchase. I think mine was about 20 bucks on Ebay, but be careful, not all eyepieces will fit all microscopes, they are all different unfortunately.

The eyepiece just pulls right out, and the reticle can be dropped in its place.

Do you think this is a small enough measurement? I don't have one, but could get this one.
AmScope EP10X23R WF10X Microscope Eyepiece with Reticle (23mm) https://www.amazon.com/dp/B005OMU8EC/ref=cm_sw_r_cp_apan_glt_fabc_3YT72BZCWYZ48AM2G89M

 

[Sue Gremlin]

You don't need to worry about how small or large the measurements are, if it fits your particular scope, it will work.
You will need to work out how much distance is between each mark with each of your objectives, this information may be in the manual it comes with since it comes with the microscope, or you may need to get yourself a 1 mm marked standard slide and do the math. I can help you with that if you need it. It seems complex but once you work that out, you don't need to do it again.

Just be careful that this reticle fits the microscope you are buying and is compatible with it. It's very easy to get it wrong.

 

[ZedikerStation]

Good news/Bad news... The reticle eyepiece will be here today.... the marked slide got delayed until tomorrow... uggh.

Anyway...... a sample from the run this morning only showed 2 of these on the whole slide where there was no air bubbles.

The problem with this slide is it was covered with air bubbles. I use the fecalizer tubes and sometimes I just get a ton of air on top, other times no air bubbles at all. What am I doing to cause so much air? I assume this slide is basically useless...