Fecal Float Slide

[ZedikerStation]

Here is my math, but I'm not sure it is correct. My answer seems very high. Would this be 25 microns?

 

[Sue Gremlin]

Ah, sorry. I have it backward.
Ok, so 39 hash marks on your reticle scale = 1 mm. This is what I am seeing in your photo. Agree?

(Micrometers = microns = μm).

So each distance between the larger marks on your reticle is about 0.04 mm, or 40 micrometers. The exact math is 39 marks/100 known (0.01 mm DIV) increments = 0.039 mm or 39.0 microns.

This stuff makes my brain bleed and leak out my ears.

For reference, a roundworm egg from a chicken is about 80 μm from end to end, and a coccidia from a chicken is about 20 μm.

 

[ZedikerStation]

Ah, sorry. I have it backward.
Ok, so 39 hash marks on your reticle scale = 1 mm. This is what I am seeing in your photo. Agree?

Agree!

So I can use your math on the next objective and just substitute the number of marks where you have 39??? Or am I over simplifying this?

 

[Sue Gremlin]

Yes! Count the number of marks on the reticle that span the known distance of 1 mm and yes, do the math the same way.

 

[ZedikerStation]

It might be starting to click..... maybe....

So the whole stage slide with all the marks is only 1 millimeter total?

 

[Sue Gremlin]

It should be. 0.01 mm per tiny line, 100 lines = 1 mm total

 

[ZedikerStation]

It should be. 0.01 mm per tiny line, 100 lines = 1 mm total

Sure looks big under the scope..... LOL

So my 10X shows 98.5 eye marks = 1.0 mm (the whole slide).

 

[ZedikerStation]

Hi @Sue Gremlin ! I'm back.

These are from this evening. We've got 1 hen a bit off and we've ruled out several things like egg bound, crop issues, etc.

These are about 50 x 75 microns at 400X

I'm going with Ascaridia. Your thoughts?

 

[ZedikerStation]

This one at 100X

 

[Sue Gremlin]

Hi! I think the eggs posted in your first post are mite eggs. Whether they are chicken mites or feed mites or environmental mites I cannot say. Just keep in mind they may not be parasitic mites.
The one at 100x is an ascaridia, great job with the ID. Note the difference in shape and the thickness of the shell of the egg between the two. The mite eggs are actually egg shaped, one point is wider than the other. The ascaridia has essentially equal ends and a thicker shell.