Has anyone had DNA sexing be wrong? Pullet with large peacomb

peachini44

Chirping
Dec 18, 2020
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I am curious if anyone has had DNA sexing be inaccurate? I took feathers at 6 weeks and this easter egger (opal legbar x a "blue egg layer") came back as female. However, at 9 weeks her comb has really been growing this last week or two and now is starting to look cockerel-ish. I am new to peacombs and only have on fast developing cockerel to compare her too (same age but he has pointed feathers including the sickle tail feathers, very red and developed pea comb, and crows since 8 weeks).

She never had a distinct full 3 rows, just the middle beads and then almost like an asymmetrical horseshoe shape of the 2 side rows. Now the rows are filling in and becoming more prominent. It still does not compare to the cockerel of the bunch, but he is very developed already. She also always had a grey-pink color face and comb color as a chick that grew pink with age, which also happened to another cuckoo female I had, so I think this is probably normal. It is still distinctly pink compared to the cockerels but it has deepened from pale to more bubblegum color. Sometimes it is more red but I think its from flushing/increased blood flow and it comes and goes. Lastly, in the last week the 4 hatch-mates have all been sorting out pecking order and sparring and she and the definite cockerel go at it the most. That being said, she has always acted more like a pullet just with a head-hen/dominant personality. Her feathers so far are all rounded too.

Curious if anyone has gotten a wrong result, and also will follow with a few pictures for gender opinion. I'd be so sad if she really isn't a she afterall because I can't keep her and she is so sweet and fun.... but would rather know sooner rather than later. I -THINK- she is a she and is just developing a bit fast, but I need some other opinions for peace of mind!
 
Yes I have had wrong dna sexing before. :oops:

mine was eggshell testing.

agree, we need photos. The three rows for pea combs is hit or miss. I think it’s more than 50/50 though at a really young age. Girls do have more as they develop, though so you may be fine.

the other sounds a bit early maturing so you may have to wait a couple of weeks to be sure on this one. Looking forward to photos.
 
Yes I have had wrong dna sexing before. :oops:

mine was eggshell testing.

agree, we need photos. The three rows for pea combs is hit or miss. I think it’s more than 50/50 though at a really young age. Girls do have more as they develop, though so you may be fine.

the other sounds a bit early maturing so you may have to wait a couple of weeks to be sure on this one. Looking forward to photos.

May I ask who you used? I used IQ Bird and feathers on her, maybe I should send in blood? My husband and I have grown so attached to her, this would be so sad.

Also, that is interesting you had it with eggshell. Was it wrong for male or female? I'm extra curious because I just got back my silkie results last night and it says only 1 female out of 6. I did eggshells for 3, and 3 blood (female was a blood sample).
 
I'll probably have to upload better ones tomorrow, but these are from tonight.
 

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I will get better body shots tomorrow unless I can find some good ones from earlier this week in my camera roll.
 

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The DNA testing is very accurate in my experience, over 99%. I have sent both feathers and eggshells to IQ genetics. It is very important to wear gloves and not contaminate the sample.
I think it is too early to tell with your lovely bird. 😊
 
I forgot I took these yesterday. Her feathers in the saddle area look just like her sister/half sister's so far. Her sister came back as pullet and has the tiniest little yellow comb...so a starke contrast to Blue's and all I have to compare to.
 

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