Well, I would save all my eggs I wanted to set @ room temperature for up to 10-14 days before viability starts to reduce. And set them at the same day and time, for future reference. If you have a separate hatcher, then I set them at least 5 days apart (giving time for late hatchers to still try) if I wanna set more eggs. That way my hatcher can stay closed for lock down. What kind of chicken eggs are you hatching? Are the dark or light? Are you using dry incubation or standard method? I know some breeds appear to be harder to hatch than others b ut haven't heard anything about ISA's or Reds. That's pretty good that you can already read your eggs at only 4 days! I am still working on technique and accidentally aborted 1 fetus on a misread candle during my last hatch. It already had eyes formed. I usually pull any I can't tell by 10 days, but give it until then because of my limited experience level. Any blanks I pull, get scrambled and fed back to my animals so there is no waste.
Setting them on the same day ensure they can click and chirp at each other and communicate to coordinate their hatch. Hatching requires different humidity than incubating. Mine is the GQF 1588, kinda the same as yours. I love it so far and use my original 1602n for the hatching.
Did your eggs go directly from the fridge to the bator and sweat inside there, or were you somehow able to bring them to room temp without sweating? It isn't recommended but I have broken plenty of the rules and still have good hatches. Seems like you are going to also be one who breaks all the "rules", and I bet you will still have a swell time. Good luck! Anyways, in nature... the hen leaves the eggs over night every time she lays until she has enough gathered to sit on. Surely it gets fridge temp some places when they do that!
Another reason to set on the same day is because chicks that hatch later will be weaker than the earlier hatching ones in my experience.
Since, like you I want to have hatching going on and I was too excited to wait for a clutch or the next clutch. Anyways, I started setting every Sunday, which is also when I ditch non starters and make room in my incubator which also holds 41. And so all hatches take place on that day as well. Of course you would wanna pick whatever day of the week would work best for you, should you decide and try that method.
Since a hen also warms them up every time she sits to lay an egg... I
might consider pulling today's set while collecting more and then set them together... I emphasized might , because I'm not really sure what I do with new information sometimes until I get put in that position.
But oh boy, you are off to an adventure! Chickens are cool, but hatching is fun!
