This is my first year seperating breeding pens, so please don't take what I say as expert; that said, following much research and advice from here, I have one roo per 5 hens in seperate pens, so far all eggs are fertile and all hens are happy.
I have one pen with a few extra hens that need to go, and I have noticed some infertile eggs coming from there; and, I have one pen with only two hens and the roo is working them pretty hard. Also, I have a back up roo as a spare for one of my harder to get breeds, he's going crazy (and driving me nuts) with no hens of his own.