HmmmI separated him after about 10 minutes because he was posturing and going after them with his spurs. They are all females.
How old is he?
It’s not unusual behavior but he will probably feel like dominating the new ones.
Separating him certainly isn’t a bad idea but not for too long as then he’ll have to be reintegrated too.
Is he being separated where the girls can still see him and he them?