Lice or Mites?

Once is not enough because nothing will kill the eggs so repeated weekly spraying is necessary. I have used a fine tooth comb to get the nits off. I would spray once a week for 3 or 4 weeks. Are you using a premixed permethrin spray? Also most poultry dust has permethrin in it.
 
Actually, they can and will! But they don't survive for more than a day, and a quick shower normally gets rid of them.
Speaking from experience 😂 those darn buggers found a way onto me and I was dusting one of the birds.
 

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