jenn-
Chirping
Does this roo's eye look like Marek's or could he just be bicolored? He has no other symptoms but I do have one sick with God knows what.
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Did you purchase your chicks from a commercial retailer or did you hatch them? The ones that I've bought as chicks have come with the vaccine.
Do you see any other signs? Lumps on the skin? Paralyzation of some kind? Extra appetite?
You should isolate them and keep a very strict biosecurity policy before and after you come to treat them(they always be the second in line, in your chores tour. change shoes before and after you treat them,always wash your hand after you treat them) until you will decide the final sedition. But if it is a question that have to be answered ASAP, where there is a doubt, there is NO doubt!
Good luck!