OK Exciting New Method!!
An accurate temperature test reveals body heat in fertile Emu eggs by day 27, probably earlier.
I used a good quality 2-mode infra red thermometer like this one:
http://www.baby4you.co.nz/shop/New+...e+design+from+Jumper.html?id=ddvf7jdD&mv_pc=s
This allows you to rest the head of the thermometer on the egg shell and take an instant temperature reading of the actual egg temperature with good accuracy.
Please note: This only works if your eggs vs. incubator arrangement has all the eggs warmed to the same degree! If one side of your incubator is warmer than the other it will be very hard. It also helps if your incubator produces more heat on the top of the egg ... mine is a single level, fan forced, with the heat and fan at the top, so the top of the egg is warmer than the bottom. This is like natural incubation.
Now the method. All temperatures in degrees centigrade (C).
Open your incubator and quickly take a temperature reading from the tops and undersides of each egg. Write these down and note any differences. Take the eggs out and lay them all on a flat surface. Wait 5 minutes. Take top and bottom temps again.
Now compare them all. All the eggs should be more or less the same temperature on top at the first reading. Within about 0.3C anyway.
Fertile eggs will be slightly warmer (Perhaps 0.5C or more) underneath at the first reading as compared to infertile eggs.
At the second reading, infertile eggs will have lost more temperature than fertile ones. Fertile eggs should only have lost about 2.5C. Infertile eggs will have lost significantly more.
The bottom reading is particularly interesting after the cooling period, as infertile eggs will be dropping temperature fast on the underside (several degrees) whereas fertile eggs only lose about 3 degrees as compared to the initial reading.
Later the difference becomes even easier to determine. I am now at day 34, and I simply check the temp top and bottom when I open the incubator. The fertile eggs are almost the same temperature (only 0.2-0.4C difference) at the botttom as at the top. The fetal body heat is carrying right through.
The infertile eggs are 1-2C lower at the bottom as compared to the top.
This method obviously works on the same principal as the hand-feel for warmth on the pointy end as opposed to the bubble end, but because it uses a digital temperature reading it is more accurate and can be used earlier.
I have confirmed it at day 27 so far: I suspect it can be used earlier still.
I would suggest that you take the top and bottom temperatures of the eggs within the first few days and record them. Using this as a baseline may help detect changes (eg bottoms of eggs slightly warmer) much earlier than just trying to compare eggs to other eggs.
I also have eggs at 10 days, but it doesn't seem to show anything with those yet. I did not take a baseline however. I will keep trying every few days and update this thread when I have a result.
Incidentally, this method works as a confirmation of life or death for late term eggs of other species too, even those that can be candled. For instance I have 2 goose eggs due in 2 days, they are looking a little late, I candled them yesterday and they were kicking, today one does not move ... I couldn't be sure if it was asleep or dead ... but comparing the temperature of the live to the still one after 5 minutes showed me a 2.8C drop in temperature on the still fetus ... which by that I assume is dead.