How many chickens do you have? Are these your first chickens? There are other respiratory diseases besides MG (mycoplasma gallisepticum.) Those include infectious bronchitis virus, MG, coryza, ILT, and others.Testing or getting a necropsy done by the state vet on a sick chicken would be the only way to know for sure what they may have had. Have you ever seen bubbles or foam in an eye, or any swelling of the face or eyelid? Was there ever any bad odor from their faces? Since Tylan has helped, it may well be MG. Sometimes they occur along with a secondary respiratory disease. There is no need to cull your whole flock if you just have a small flock. The eggs are always fine to use, but MG can pass through hatching eggs. So just don’t sell eggs that someone is going to hatch. Treat any sick birds with the Tylan, and if you should lose one or one is very sick, send it in to the state vet for a necropsy. MG only lives for about 3 days on equipment, clothing, shoes, hair, or in your environment, so one day if you lose these birds, you can get healthy chicks and start over. They may live for many years though, and lay lots of eggs.