Hi I had two hens that died within two weeks of unknown causes. With the second hen we had necropsy done at UC Davis and we got few preliminary reports and one final report sent to us. They all say pretty much the same thing. It is almost confusing to read and it sounds like totally foreign language to me and we were wondering if there is some expert who could explain to us what exactly happened and what we need to do now. As much as I understand there was some infection going on and I can just assume that the first hen probably died of the same thing therefore my other hens might be also infected. So I think that I might need to preventively treat my remaining hen so they don't die of the same thing. Also the report says that the hen had abundant body fat. Does that mean my hen was too fat that could cause some of the the illnesses? How much are you suppose to feed the hens? We gave them unlimited chicken feed and every morning they also got small half head of lettuce and 1-2 tomatoes. That doesn't seem to me like it should get them fat. Please let me know what you think. thank you Laboratory Findings/Diagnosis Final Report: 1. Tracheobronchitis, lymphoplasmacytic, lymphofollicular, moderate, indeterminate Mycoplasma synoviae PCR result. 2. Serositis, heterophilic with egg yolk protein, egg yolk peritonitis, Gallibacterium anatis biovar haemolytica isolated. 3. Hepatic lipidosis. Case Summary 08/23/11: All tests are now completed and the results remain the same as previously reported. 08/18/11: The cause for the tracheobronchitis is uncertain but a Mycoplasma infection remains a possibility. The bacterial cultures from the lung and trachea were unremarkable. The Mycoplasma PCR from Mycoplasma synoviae was not definitive but Mycoplasma synoviae infection remains a likely cause for the respiratory lesioins but this can not be confirmed. The serositis appears to be related to the egg yolk protein and a bacterial infection caused by Gallibacterium anatis biovar haemolytica which was isolated from the coelomic cavity. A final will be sent when the rest of the tests are completed. 08/16/11: In the respiratory tract the lesions in the trachea and to a lesser degree, the major bronchi of the lung, are suggestive of an infectious etiology. Tests are in progress in an attempt to identify the cause. Mycoplasma infection would be a possibility. The gross and histologic changes in the coelomic cavity are consistent with so -called egg yolk peritonitis and it appears that there has been a secondary bacterial infection. Cultures are pending on this. Another report will be forthcoming. Gross Observation Examined was an adult hen that was in fair postmortem condition. The animal has abundant body fat. Internally, the ovary has multiple 2-3 cm diameter ova. The oviduct is large, but empty and grossly unremarkable. There is a small amount of yellow sticky semifluid material in the coelomic cavity surrounding the oviduct and ovary. The liver is slightly enlarged and friable with a yellow-brown discoloration, consistent with fat accumulation. The spleen is grossly unremarkable. In the respiratory tract, the trachea contains excess clear to slightly gray mucoid exudate and the tracheal mucosa is diffusely congested. The lungs are moderately congested with equivocal red areas of consolidation bilaterally on the dorsolateral margins of the lung. The air sacs are grossly unremarkable. No remarkable gross lesions were noted in the infraorbital sinuses. No remarkable gross lesions were noted in the brain or peripheral nerves. In the crop there is grayish -green fluid with roughage. The proventriculus contains a small amount of feed material. The ventriculus contains grit and feed material. The small intestine contains a small amount of greenish-brown fluid content. The ceca contained pasty greenish-brown content. The colon is empty. No remarkable gross lesions are noted in the kidneys. Histology Histologic examinations were performed on samples of brain, peripheral nerve, trachea, lung, heart, liver, kidney, spleen, ovary, oviduct, crop, proventriculus, ventriculus, small intestine, pancreas, cecum and skeletal muscle. The tissues are in fair postmortem condition. Significant histologic lesions will be summarized. No remarkable histological lesions were identified in the brain or peripheral nerve. In multiple sections of trachea examined, there is multifocal mucosal infiltrate by lymphocytes and plasma cells with occasional lymphofollicular aggregates. The tracheal epithelium is intact. There is mucus on the surface. In the lung, there is mild lymphoplasmacytic peribronchial infiltrate. In the liver, there is marked cytoplasmic vacuolization of centrilobular and mid zonal hepatocytes , consistent with lipidosis. The spleen has lymphofollicular hyperplasia. In the proventriculus, there is mucosal lymphoplasmacytic infiltrate in the mucosa. In the sections of the ventriculus, small intestine, cecum and oviduct, there is an accumulation of eosinophilic globular material, interpreted as egg yolk protein, accumulating on the surface with multifocal areas of heterophilic exudation associated with bacterial colonization. The serosa is expanded with edema fluid and mixed inflammatory cell infiltrate. No other remarkable histologic lesions are identified.