I can get useful information within a single clutch of 10. Hybrids reared in mixed group and pures reared in pure. Enough have been done here to indicate the games have better / more effective sperm than the doms. The game sperm dominates after only a couple days while dom semen does not dominate until the game sperm has aged a few days. To get that information knowledge of lay sequence must be related to chicks at hatch which is where pedigree baskets come in.