I've got a batch in right now that were laid in an outdoor coop and it's ranged from 25-40 degrees, so I'll mark down what develops and what comes out. I seem to remember I did refrigerate some on purpose a couple years ago but didn't end up tallying males, might have been one of my bad hatches.Now you've got me curious..lol.. going to have to try this some time, especially if it will help to weed out roosters, I don't want to have more than 2 in there if I can help it.