My flock does this after an egg is laid, when they are startled, or when I start it first 

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For sure! Mine are sissiesI have some hens who will do this after laying an egg even if there is nobody and nothing around to scare her... while there are others who remain silent even when the cat comes into the coop and freaks her out. So while I've always heard that they are alarm calls, I find that my chickens get alarmed at not-too-alarming things... like strange lighting. Or a windy day. Or a rain storm. Or even their fellow hens coming to check on them.
Yes it is just like you say, I came to knowledge that chickens are sort of terottorial, as soon as something enters their comfort zone thay are unhappyFor sure! Mine are sissiesMy rabbits (free range) Constantly hop around and startle them. When I first put a rabbit in a pen in the coop, I had a hen freak out (egg songing) for 45 minutes straight.
Make recordings of egg song, call hens make following the laying of an egg and leaving nest, and the distressed group cackling. You should be able to distinguish them. Even my dogs can. The distressed group cackling even when played on computer gets my dogs at least a little riled while the other calls do not. The chickens would also respond differently if recordings played to them.
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Here is a group song, after a very first egg (Barred Rock hen)
EDIT: TURN VOLUME DOWN! Lol
I was sitting in there with them taking photos when she had jumped out of the nest box and started the chaos, so I am 100% sure it is related to egg laying. It was her very first egg, and she may have been a little overly proud. The silkies in the video always took the song to the next level. The one I focused on a lot was the one that egg songed for 45 minutes after being introduced to a miniature rabbitWOW... see, but that sounds like an alarm call to me. I will have to figure out how to record video of my chickens and get the chorus egg song I hear, which is a little different but definitely just as loud.