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Did the culling of those 4 other roos change the dynamics completely? Why, all of a sudden, do I have one that is scared to death to be in the coop with the others when there never was an issue before? Any advice greatly appreciated... I have absolutely no idea what the heck happened when they were all best friends a few days ago.
Chances are, Yes. Changes in flock members will change the dynamics - sometimes rather radically. Most of the time once a new pecking order is established, things calm down again. As long as she is not being injured, leave the hen in there until they work in out amongst themselves. (It can be hard to watch... but chickens have been doing this for thousands of years... )