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Yesterday you said the colony has open brood, so there was a queen in there less than 2 weeks ago. No such thing as "older brood" it's all capped by 8 days after the egg is laid. If in that time a virgin queen emerged, she will kill her mother and go on mating flights. Bees won't go laying worker with open brood. If you try and combine now before the new queen has completed mating flights, she may kill the queen you combined with. Then you could be left with 2 queen less colonies. I would wait until the weekend.
It had larvae that was about 7 days old. The only large cell that I saw was this one. I touched it with my hive tool, that is the rough spot you see on it. Hopefully we will have a new queen, but I didn't see any other possible queen cells. I didn't see this cell on our last inspection, but I didn't pull all of the frames out, because I didn't want to take a chance of killing our queen. I saw eggs, larvae, capped brood and lots of bees. I will trust your advice and we will wait until the weekend. Thank you!
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My youngest queen, only 3 1/2 weeks old and has 3 frames of open winter brood already.
View attachment 4213929
She's beautiful and looks like she's laying a strong pattern.
 
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