Quote: There is an interesting lecture about incubation given by U of A Dr Keith Bramwell:
that has some outstanding content. Audio is so-so.
At about 13 minutes he starts talking about fertilization in the hen and it continues until about 20 minutes.
The first roo's sperm is not removed but covered over by the new rooster's sperm in the hen.
In a nutshell, he tells us that the hen has sperm-storage tubules that act as a reservoir for the rooster's sperm. The sperm that goes in first is the last to be removed/used. Example experiment: If you have a white hen with a white rooster (expect white chicks) then remove him and place in a black rooster (expect black chicks) you will get black chicks from eggs collected starting on the second day from putting the new rooster in if that black rooster is observed mating with the hen.
So in this case, if the CL rooster initially bred the hen and his sperm covered over the SS's sperm in the tubule but the SS sperm is still viable. If the CL stopped breeding the hen for a few days, the reserved SS sperm would then fertilize the eggs at that time until another roo bred the hen or until the SS sperm was exhausted. Alternatively, if the CL was unsuccessful at breeding her for some reason (fertility problem, inexperience), the SS sperm will still be there to fertilize eggs until the sperm is used up or until covered over by another roosters sperm.
One person on the video in the audience states that he had a hen that was not exposed to a roo and she laid a fertile egg at 60 days from last exposure.