Eggs are not hatching

[Pyxis]

I’ve kept the temperature at 99.9-100 the whole time. I have not been opening the dead ones

Have you been measuring the humidity?

When you say “lockdown” what are you referring to

Lockdown is the last three days of incubation, when you increase the humidity and stop turning the eggs.

 

[HuskerHens18]

@Bradleyb Lockdown is when you stop turning the eggs and raise the humidity on day 18 of your incubation.

 

[Bradleyb]

I may not have raised the humidity enough.

If I have 2 different batches of eggs in the same incubator that hatch 2 weeks apart, will it hurt the new batch to raise the humidity for the hatching eggs?

 

[ChickenCanoe]

What's your tempature and humidity at? At lockdown, humidity should be between 65°F and 75°F.

You mean 65% to 75% RH

 

[ChickenCanoe]

I may not have raised the humidity enough.

If I have 2 different batches of eggs in the same incubator that hatch 2 weeks apart, will it hurt the new batch to raise the humidity for the hatching eggs?

It won't hurt to raise the humidity for a few days. It is overall moisture loss throughout that is the key concern.

 

[HuskerHens18]

I may not have raised the humidity enough.

If I have 2 different batches of eggs in the same incubator that hatch 2 weeks apart, will it hurt the new batch to raise the humidity for the hatching eggs?

For staggered hatches(which is when you have eggs that are days/weeks apart) you should have two incubators. The new ones probably won't make it if you bump up the humidity and stop turning them. But your hatching eggs won't hatch if the humidity is too low. I'm not real sure what to tell you to do. Hopefully some experts will give you better advice for a successful staggered hatch

 

[ChickenCanoe]

Staggered hatches aren't ideal but doable.
Humidity isn't a set number.
Ambient humidity changes all the time in nature and a setting hen can't control that.
It has been raining here for days. Humidity has been between 95 and 100%. A hen can't lower that. When we have a dry spell, it can hover around 35% even though this isn't an arid climate.
When hatching is imminent, a hen sits tight holding precious humidity in the nest environment.
What you are trying to achieve with artificial incubation is a goal of about 13% weight loss throughout for chicken eggs. IMHO, it doesn't matter if that weight loss is all in the course of a few days or week or spread out - as in nature.
The other thing you want to achieve is enough humidity when eggs pip to prevent shrink wrapping.
As was mentioned above, turning is a different issue. As long as turning is frequent the first week to 10 days, you can miss a couple days when the first batch is hatching.
'Lockdown' isn't set in stone either. It is preferable to raise humidity and not open the incubator the last 3 days (in case some pip early) but usually not necessary. If none are pipping in your first batch, you can continue to turn the newer eggs. Just make sure you get the humidity back up by filling all reservoirs or whatever means you have.

 

[ChickenCanoe]

I’ve kept the temperature at 99.9-100 the whole time. I have not been opening the dead ones

According to your thermostat and thermometer. Until you calibrate or verify accuracy with a guaranteed accurate thermometer, it is a guess because we know that the majority are inaccurate out of the box. Go to a hardware store and look at their thermometers. Invariably, most won't agree with each other and some will be way off..
Did the chick that hatched out of the 10 eggs hatch early, late or right on time?