They will stay on the coop floor as long as they feel safe there but, as with most new things, at some point one will hop up there and the rest will just follow.
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Yes, you should be blocking the nest box until you get an egg but only because they will get in the habit of sleeping and pooping there and later you will wind up with poopy (or broken) eggs. Your hens can cuddle on the coop floor until one decides to try out the roost and the rest will just follow along. There isn't a magic age for this but it almost always happens before they start to lay.All of my hens are cuddling together in the nesting box. These are my first hens, I have had them for less than a week. Should I be blocking the nesting boxes so they can't cuddle?