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If it works, what's the harm? Better than having a bunch of naked chickens running around. In nature, if a chicken can find a tuna, or any fish, it will eat it. People forget that their delicate lttle coop chickens are in fact scavengers. There is little that they can't/won't eat. The OP fed tuna as a protein supplement. It is not unheard of to give fish meal to chickens as a protein supplement. BTW, I have fed a nice high protein balanced feed to my layers for years and on occasion still had trouble with feather picking. So next time I will try Forco. Considering all the stuff chickens will eat if given the opportunity, I cannot imagine anything in a horse feed that could possibly harm their delicate digestion.Looking at Forco's website, this product looks to be a probiotic. Avians need a specific type of probiotic, giving a horse probiotic to avians is not a good idea. This horse stuff may be doing internal damage to birds. Forco's website also says "There are no supplemental vitamins, minerals or proteins". In nature chickens never eat tuna, why would you give it to them. The chickens may have had issues trying to digest the protein from fish. Feather plucking is a protein deficiency, there is protein in feathers, that is why chickens eat them. Try something more suited for avains, like game bird feed, it is 24% protein instead of layer feed that is 14-16% protein. Or mealworms, around 50% protein.