Shouldn't. At least it never has in mine. I've only had it go that low a couple times, but w/in 5 minutes it was back up over 60 and headed to my safe zone...(above 70%).I removed the other two hellians and put them in the brooder. I ended up helping the little one because it was not progressing and you could see it panting because of exhaustion. Now its peeping and sleeping in the incubator. There was a little blood but it stopped. Its not able to stand up yet. I am still waiting for the other 9 to do something. The humidity did drop to 50% but was right back kline about 5 mins. Will this affect the others trying to hatch?